Recombinant Human PDE6D Full length protein, His-tagged

• Cat.No.: PDE6D-301348H
• Product Overview: Recombinant Human PDE6D protein(1-150 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: 1-150 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AASequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 μg/μl in 200 μl sterile water for short-term storage.
  After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: PDE6D phosphodiesterase 6D, cGMP-specific, rod, delta [ Homo sapiens ]
• Official Symbol: PDE6D
• Synonyms: PDE6D; phosphodiesterase 6D, cGMP-specific, rod, delta; retinal rod rhodopsin-sensitive cGMP 3,5-cyclic phosphodiesterase subunit delta; protein p17; GMP-PDE delta; PDED
• Gene ID: 5147
• mRNA Refseq: NM_002601
• Protein Refseq: NP_002592
• MIM: 602676
• UniProt ID: O43924