Recombinant Human PDE5A, GST-tagged, Active

• Cat.No.: PDE5A-479H
• Product Overview: Recombinant human PDE5A (537-end) was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 63 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 537-end a.a.
• Description: PDE5A is a member of the cyclic nucleotide phosphodiesterase family. PDE5A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5"-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. PDE5A regulates nitric oxide (NO)-induced relaxation and cGMP breakdown in smooth muscle cells in vas deferens. PDE5A expression predisposes mice to adverse left ventricular remodeling after myocardial infarction. Furthermore, increased myocardial PDE5A expression in patients with advanced cardiomyopathy can contribute to the development of heart failure.
• Sequence: 537-end.
• Applications: PDE Assay.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PDE5A phosphodiesterase 5A, cGMP-specific [ Homo sapiens ]
• Synonyms: PDE5A; phosphodiesterase 5A, cGMP-specific; CN5A; PDE5; PDE5A1; CGB-PDE; cGMP-binding cGMP-specific 3",5"-cyclic nucleotide phosphodiesterase; EC 3.1.4.35; cGMP-binding cGMP-specific phosphodiesterase; phosphodiesterase 5A; cGMP-specific 3",5"-cyclic phosphodiesterase
• Gene ID: 8654
• mRNA Refseq: NM_001083
• Protein Refseq: NP_001074
• MIM: 603310
• UniProt ID: O76074
• Chromosome Location: 4q25-q27
• Pathway: Progesterone-mediated oocyte maturation; Purine metabolism
• Function: 3",5"-cyclic-GMP phosphodiesterase activity; cGMP binding; hydrolase activity; magnesium ion binding; nucleotide binding; zinc ion binding