Recombinant Human PDE4D, GST-tagged, Active

• Cat.No.: PDE4D-478H
• Product Overview: Recombinant human PDE4D (225-end) was expressed by baculovirus inSf9insect cells using an N-terminal GST tag. MW = 82 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 225-end a.a.
• Description: PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the "dunce" gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFα upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFα treatment and increased by 24 hours. Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility.
• Sequence: 225-end.
• Applications: PDE Assay.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PDE4D phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) [ Homo sapiens ]
• Synonyms: PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); DPDE3; STRK1; HSPDE4D; PDE4DN2; FLJ97311; DKFZp686M11213; phosphodiesterase 4D; dunce-like phosphodiesterase E3; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; EC 3.1.4.17; phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3); cAMP-specific 3",5"-cyclic phosphodiesterase 4D; PDE43; phosphodiesterase 4D, cAMP-specific (dunce; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Gene ID: 5144
• mRNA Refseq: NM_001104631
• Protein Refseq: NP_001098101
• MIM: 600129
• UniProt ID: Q08499
• Chromosome Location: 5q12
• Pathway: Progesterone-mediated oocyte maturation; Purine metabolism
• Function: 3",5"-cyclic-AMP phosphodiesterase activity; hydrolase activity; metal ion binding