Active Recombinant Human PDE4D protein, GST-tagged

• Cat.No.: PDE4D-10H
• Product Overview: Recombinant Human PDE4D(2–507 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 2-507 a.a.
• Description: This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol and 16 mM Glutathione.
• Bio-activity: ≥ 13,600 pmol/min/µg
• Molecular Mass: 84 kDa
• Purity: > 86%
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37 centigrade.
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Storage: >6 months at -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.27 mg/ml

Gene Information

• Gene Name: PDE4D phosphodiesterase 4D, cAMP-specific [ Homo sapiens ]
• Official Symbol: PDE4D
• Synonyms: PDE4D; phosphodiesterase 4D, cAMP-specific; DPDE3, phosphodiesterase 4D, cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); cAMP-specific 3,5-cyclic phosphodiesterase 4D; phosphodiesterase E3 dunce homolog (Drosophila); cAMP-specific phosphodiesterase PDE4D6; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); DPDE3; PDE43; STRK1; HSPDE4D; PDE4DN2; FLJ97311; DKFZp686M11213
• Gene ID: 5144
• mRNA Refseq: NM_001197221
• Protein Refseq: NP_001184150
• MIM: 600129
• UniProt ID: Q08499
• Chromosome Location: 5q12
• Pathway: DARPP-32 events, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem
• Function: 3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; cAMP binding; drug binding; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity