Active Recombinant Human PDCD1 protein, His-tagged

• Cat.No.: PDCD1-173H
• Product Overview: Recombinant Human PDCD1 protein(Q15116-1)(Leu 25 - Leu 288), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu 25 - Leu 288
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH8.0 with detergent. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-1 Full Length, His Tag at 0.2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.4-3 ng/mL.
• Molecular Mass: The protein has a calculated MW of 30.7 kDa. The protein migrates as 40-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >80% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116