Recombinant Human PCNA, His-tagged

Cat.No. : PCNA-175H
Product Overview : Recombinant Human PCNA (Met 1-Ser 261) fused with a polyhistidine tag at the N-terminus, was expressed in Baculovirus-Insect cells.
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Species : Human
Source : Insect Cells
Tag : Non
Protein Length : 1-261 a.a.
Description : The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Molecular Mass : The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
Predicted N terminal : Met
Purity : > 90 % as determined by SDS-PAGE
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Formulation : Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10%glycerol, pH 7.0, 2mM DTT
Storage : Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
OfficialSymbol : PCNA
Gene Name PCNA proliferating cell nuclear antigen [ Homo sapiens ]
Synonyms MGC8367; PCNA; Proliferating cell nuclear antigen; Cyclin; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
Gene ID 5111
mRNA Refseq NM_002592
Protein Refseq NP_002583
MIM 176740
UniProt ID P12004
Chromosome Location 20pter-p12
Pathway Base excision repair; Cell cycle; DNA replication; Mismatch repair; Nucleotide excision repair; Cell Cycle, Mitotic; DNA Repair; DNA Replication; Telomere Maintenance
Function DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity

Not For Human Consumption!

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