Recombinant Human PCNA, His-tagged
Cat.No. : | PCNA-175H |
Product Overview : | Recombinant Human PCNA (Met 1-Ser 261) fused with a polyhistidine tag at the N-terminus, was expressed in Baculovirus-Insect cells. |
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Species : | Human |
Source : | Insect Cells |
Tag : | Non |
Protein Length : | 1-261 a.a. |
Description : | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. |
Molecular Mass : | The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions. |
Predicted N terminal : | Met |
Purity : | > 90 % as determined by SDS-PAGE |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation : | Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10%glycerol, pH 7.0, 2mM DTT |
Storage : | Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | A hardcopy of COA with reconstitution instruction is sent along with the products. |
OfficialSymbol : | PCNA |
Gene Name | PCNA proliferating cell nuclear antigen [ Homo sapiens ] |
Synonyms | MGC8367; PCNA; Proliferating cell nuclear antigen; Cyclin; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein |
Gene ID | 5111 |
mRNA Refseq | NM_002592 |
Protein Refseq | NP_002583 |
MIM | 176740 |
UniProt ID | P12004 |
Chromosome Location | 20pter-p12 |
Pathway | Base excision repair; Cell cycle; DNA replication; Mismatch repair; Nucleotide excision repair; Cell Cycle, Mitotic; DNA Repair; DNA Replication; Telomere Maintenance |
Function | DNA binding; DNA polymerase processivity factor activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity |
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Not For Human Consumption!
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