Recombinant Human PAWR protein, His & GST-tagged

• Cat.No.: PAWR-538H
• Product Overview: Recombinant Human PAWR aa. (Gly48~Gln385) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Gly48~Gln385
• Description: The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.
• Form: Freeze-dried powder
• Molecular Mass: 66kDa as determined by SDS-PAGE reducing conditions
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 8.6.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PAWR pro-apoptotic WT1 regulator [ Homo sapiens (human) ]
• Official Symbol: PAWR
• Synonyms: PAWR; pro-apoptotic WT1 regulator; PAR4; Par-4; PRKC apoptosis WT1 regulator protein; PRKC, apoptosis, WT1, regulator; WT1-interacting protein; prostate apoptosis response 4 protein; prostate apoptosis response protein 4; prostate apoptosis response protein 4; prostate apoptosis response-4; transcriptional repressor PAR4
• Gene ID: 5074
• mRNA Refseq: NM_002583.2
• Protein Refseq: NP_002574.2
• UniProt ID: Q96IZ0