Recombinant Human PARP2, His-tagged

• Cat.No.: PARP2-7559H
• Product Overview: Recombinant human PARP2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Antigen Description: PARP2 is poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Molecular Mass: 42.5 kDa (376aa)
• Protein length: 233-583aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMQLDLR VQELIKLICN VQAMEEMMME MKYNTKKAPL GKLTVAQIKA GYQSLKKIED CIRAGQHGRA LMEACNEFYT RIPHDFGLRT PPLIRTQKEL SEKIQLLEAL GDIEIAIKLV KTELQSPEHP LDQHYRNLHC ALRPLDHESY EFKVISQYLQ STHAPTHSDY TMTLLDLFEV EKDGEKEAFR EDLHNRMLLW HGSRMSNWVG ILSHGLRIAP PEAPITGYMF GKGIYFADMS SKSANYCFAS RLKNTGLLLL SEVALGQCNE LLEANPKAEG LLQGKHSTKG LGKMAPSSAH FVTLNGSTVP LGPASDTGIL NPDGYTLNYN EYIVYNPNQV RMRYLLKVQF NFLQLW
• Purity: >85% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: PARP2 poly (ADP-ribose) polymerase 2 [ Homo sapiens ]
• Official Symbol: PARP2
• Synonyms: PARP2; poly (ADP-ribose) polymerase 2; ADP ribosyltransferase (NAD+; poly(ADP ribose) polymerase) like 2 , ADPRTL2, poly (ADP ribose) polymerase family, member 2; poly [ADP-ribose] polymerase 2; ADPRT-2; hPARP-2; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 2; poly[ADP-ribose] synthetase 2; NAD(+) ADP-ribosyltransferase 2; poly (ADP-ribosyl) transferase-like 2; poly (ADP-ribose) polymerase family, member 2; ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
• Gene ID: 10038
• mRNA Refseq: NM_001042618
• Protein Refseq: NP_001036083
• MIM: 607725
• UniProt ID: Q9UGN5
• Chromosome Location: 14q11.2-q12
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Regulation of Telomerase, organism-specific biosystem
• Function: DNA binding; NAD+ ADP-ribosyltransferase activity; transferase activity, transferring glycosyl groups