Recombinant human pancreatic lipase Reference Standard

• Cat.No.: PNLIP-13H
• Product Overview: Each sample is in lyophilised form and equivalent to about 1 mL of a solution of purified recombinant pancreatic lipase from v79-rHPL cell line. The preparation has been stabilised by incorporation in a matrix consisting of Tris 20 mmol, pH=7.6 and BSA 40 g/l. No contamination, as assessed by measurement of their catalytic activity, has been detected for the following enzymes: ALP, ALT, α-Amylase, AST, esterase, GGT, and LDH. The material is kept under dry nitrogen in neutral clear glass ampoules, sealed by fusion. The residual water mass fraction of the sample had a mean value of 0.114%. It is not recommended that a portion of the lyophilised material contained in an ampoule be used.

Specification

• Species: Human
• Tag: Non
• Description: This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas.
• Usage: To make it ready for use, the material has to be reconstituted according to the procedure described in section 12 of the certification report. The intended use of the material is: a) to ensure the transferability of results obtained with procedures having the same analytical specificity; b) to assign values to secondary materials and c) to calibrate the colorimetric routine procedure, described in the report. The commutability of the material with routine in vitro diagnostic devices has not been assessed. If the material is used for the calibration of in vitro diagnostic devices the commutability has to be assessed by the user.
• Quality Control Test: Titrimetric standardized operating procedure at 37 °C (details specified in the report).
• Notes: The recombinant enzyme material was prepared from V79-rHPL cell line. V79 (Chinese Hamster Fibroblasts) have been stably transfected with human prancreatic lipase cDNA under the control of CMV promoter. However, the product must be handed as any material of human origin. It is intended for in vitro analysis only and usual laboratory safety precautions apply.
• Storage: Upon arrival at the laboratory the material shall be stored at -20 °C.However, the European Commission cannot be held responsible for changes that happen during storage of the material at the customer's premises, especially of opened samples.
• Concentration: Human Pancreatic Lipase: 17.4 ukat/L

Gene Information

• Gene Name: PNLIP pancreatic lipase [ Homo sapiens ]
• Official Symbol: PNLIP
• Synonyms: PNLIP; pancreatic lipase; pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase; PL; PTL; PNLIPD
• Gene ID: 5406
• mRNA Refseq: NM_000936
• Protein Refseq: NP_000927
• MIM: 246600
• UniProt ID: P16233
• Chromosome Location: 10q25.3
• Pathway: Acylglycerol degradation, organism-specific biosystem; Acylglycerol degradation, conserved biosystem; Digestion of dietary lipid, organism-specific biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem
• Function: hydrolase activity; retinyl-palmitate esterase activity; triglyceride lipase activity