Recombinant Human PAM Protein, His-tagged

• Cat.No.: PAM-2542H
• Product Overview: Recombinant Human PAM Protein (Phe21-Cys288) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Phe21-Cys288
• Description: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.7 kDa
  Accurate Molecular Mass: 34 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PAM peptidylglycine alpha-amidating monooxygenase [ Homo sapiens (human) ]
• Official Symbol: PAM
• Synonyms: PAM; peptidylglycine alpha-amidating monooxygenase; peptidyl-glycine alpha-amidating monooxygenase; PAL; peptidyl alpha hydroxyglycine alpha amidating lyase; peptidylglycine alpha hydroxylating monooxygenase; PHM; peptidylamidoglycolate lyase; peptidylglycine 2-hydroxylase; peptidyl alpha-amidating enzyme; peptidylglycine alpha-hydroxylating monooxygenase; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; pancreatic peptidylglycine alpha-amidating monooxygenase
• Gene ID: 5066
• mRNA Refseq: NM_000919
• Protein Refseq: NP_000910
• MIM: 170270
• UniProt ID: P19021