Recombinant Rat Pam Protein, His-tagged

• Cat.No.: Pam-1169R
• Product Overview: Recombinant Rat Pam Protein (Phe36-Val820) with His tag was expressed in 293F.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Phe36-Val820
• Description: Enables several functions, including metal ion binding activity; peptidylamidoglycolate lyase activity; and peptidylglycine monooxygenase activity. Involved in several processes, including animal organ development; fatty acid primary amide biosynthetic process; and peptide amidation. Located in several cellular components, including perikaryon; secretory granule membrane; and trans-Golgi network. Biomarker of hypothyroidism. Orthologous to human PAM (peptidylglycine alpha-amidating monooxygenase).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 89.5 kDa; Accurate Molecular Mass: 88 kDa
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Pam peptidylglycine alpha-amidating monooxygenase [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Pam
• Synonyms: PAM; peptidylglycine alpha-amidating monooxygenase; peptidyl-glycine alpha-amidating monooxygenase
• Gene ID: 25508
• mRNA Refseq: NM_013000
• Protein Refseq: NP_037132
• UniProt ID: P14925