Recombinant Human PAK4, GST-His

• Cat.No.: PAK4-723H
• Product Overview: Recombinant Human PAK4, full length, amino acids M1-R591, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells. MW = 93,968 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-591 a.a.
• Description: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PAK4 was confirmed as PAK4 by by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany)
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.154 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 3 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Full Length: Full L.

Gene Information

• Gene Name: PAK4 p21 protein (Cdc42/Rac)-activated kinase 4 [ Homo sapiens ]
• Synonyms: PAK4; p21 protein (Cdc42/Rac)-activated kinase 4; p21-activated kinase 4; p21(CDKN1A)-activated kinase 4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; Serine/threonine-protein kinase PAK 4; KIAA1142; EC 2.7.11.1; PAK-4
• Gene ID: 10298
• mRNA Refseq: NM_001014831
• Protein Refseq: NP_001014831
• MIM: 605451
• UniProt ID: O96013
• Chromosome Location: 19q13.2
• Pathway: Axon guidance; ErbB signaling pathway; Focal adhesion; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity