Recombinant Human PAICS, His-tagged

• Cat.No.: PAICS-135H
• Product Overview: PAICS, 1-425aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-425aa
• Description: PAICS, also known as phosphoribosylaminoimidazole carboxylase, is an enzyme involved in nucleotide biosynthesis and in particular in purine biosynthesis. It catalyzes the conversion of 5"-phosphoribosyl-5-aminoimidazole(AIR) into 5"-phosphoribosyl-4-carboxy-5-aminoimidazole (CAIR) as described in the reaction. Recombinant human PAICS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
• Molecular Mass: 49.5 kDa (448aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATAEVL NIGKKLYEGK TKEVYELLDS PGKVLLQSKD QITAGNAARK NHLEGKAAIS NKITSCIFQL LQEAGIKTAF TRKCGETAFI APQCEMIPIE WVCRRIATGS FLKRNPGVKE GYKFYPPKVE LFFKDDANND PQWSEEQLIA AKFCFAGLLI GQTEVDIMSH ATQAIFEILE KSWLPQNCTL VDMKIEFGVD VTTKEIVLAD VIDNDSWRLW PSGDRSQQKD KQSYRDLKEV TPEGLQMVKK NFEWVAERVE LLLKSESQCR VVVLMGSTSD LGHCEKIKKA CGNFGIPCEL RVTSAHKGPD ETLRIKAEYE GDGIPTVFVA VAGRSNGLGP VMSGNTAYPV ISCPPLTPDW GVQDVWSSLR LPSGLGCSTV LSPEGSAQFA AQIFGLSNHL VWSKLRASIL NTWISLKQAD KKIRECNL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PAICS phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase [ Homo sapiens ]
• Official Symbol: PAICS
• Synonyms: PAICS; phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase; PAIS; multifunctional protein ADE2; ADE2H1; AIRC; AIR carboxylase; SAICAR synthetase; multifunctional protein ADE2H1; ADE2; MGC1343; MGC5024; DKFZp781N1372
• Gene ID: 10606
• mRNA Refseq: NM_001079524
• Protein Refseq: NP_001072992
• MIM: 172439
• UniProt ID: P22234
• Chromosome Location: 4q12
• Pathway: Inosine monophosphate biosynthesis, PRPP + glutamine =>IMP, organism-specific biosystem; Inosine monophosphate biosynthesis, PRPP + glutamine => IMP, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem
• Function: 5-(carboxyamino)imidazole ribonucleotide mutase activity; ATP binding; identical protein binding; ligase activity; lyase activity; nucleotide binding; phosphoribosylaminoimidazole carboxylase activity; phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; protein binding