Recombinant Human P4HTM protein, His-tagged

• Cat.No.: P4HTM-3370H
• Product Overview: Recombinant Human P4HTM protein(218-563 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 218-563 aa
• AA Sequence: NGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGPGDHWVIPVSPWEHPQLGTCSVPPLPYSYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: P4HTM prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) [ Homo sapiens ]
• Official Symbol: P4HTM
• Synonyms: P4HTM; prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum); transmembrane prolyl 4-hydroxylase; EGLN4; FLJ20262; HIFPH4; hypoxia inducible factor prolyl 4 hydroxylase; P4H TM; PH 4; PH4; PHD4; Prolyl hydroxlase domain containing 4; HPH-4; HIF-PH4; proline 4-hydroxylase; HIF-prolyl hydroxylase 4; P4H with transmembrane domain; Prolyl hydroxlase domain-containing 4; hypoxia-inducible factor prolyl 4-hydroxylase; hypoxia-inducible factor prolyl hydroxylase 4; PH-4; P4H-TM
• Gene ID: 54681
• mRNA Refseq: NM_177938
• Protein Refseq: NP_808807
• MIM: 614584
• UniProt ID: Q9NXG6