Recombinant Human OTUD7B Protein, His-tagged

• Cat.No.: OTUD7B-2535H
• Product Overview: Recombinant Human OTUD7B Protein (Leu183-Met365) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Leu183-Met365
• Description: Enables Lys48-specific deubiquitinase activity and thiol-dependent deubiquitinase. Involved in several processes, including negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of macromolecule metabolic process; and protein deubiquitination. Located in cytoplasm and nucleus.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.2 kDa
  Accurate Molecular Mass: 26 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: OTUD7B OTU domain containing 7B [ Homo sapiens (human) ]
• Official Symbol: OTUD7B
• Synonyms: OTUD7B; OTU domain containing 7B; ZA20D1, zinc finger, A20 domain containing 1; OTU domain-containing protein 7B; CEZANNE; zinc finger protein Cezanne; zinc finger, A20 domain containing 1; cellular zinc finger anti-NF-kappaB Cezanne; zinc finger A20 domain-containing protein 1; cellular zinc finger anti-NF-kappa-B protein; ZA20D1
• Gene ID: 56957
• mRNA Refseq: NM_020205
• Protein Refseq: NP_064590
• MIM: 611748
• UniProt ID: Q6GQQ9