Recombinant Mouse Otud7b Protein, Myc/DDK-tagged

• Cat.No.: Otud7b-4629M
• Product Overview: Purified recombinant protein of mouse full-length OTU domain containing 7B (Otud7b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B Negatively regulates mucosal immunity against infections Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 Mediates deubiquitination of EGFR. Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 92 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Otud7b OTU domain containing 7B [ Mus musculus (house mouse) ]
• Official Symbol: Otud7b
• Synonyms: OTUD7B; OTU domain containing 7B; zinc finger, A20 domain containing 1; TRAF binding domain containing 2; Za20d1; CEZANNE; Trabid2; AI462125; 2900060B22Rik; 4930463P07Rik
• Gene ID: 229603
• mRNA Refseq: NM_001025613
• Protein Refseq: NP_001020784
• UniProt ID: B2RUR8