Recombinant Human ONECUT2 HEK293T cell lysate

Cat.No. : ONECUT2-19HCL
Product Overview : Human ONECUT2 over-expression lysate was derived from Human HEK293T cell line.
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Description : This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation.
Source : Human HEK293T cell line
Species : Human
Tag : Myc&DDK
Form : The cell lysate is provided in RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
Molecular Mass : 54.1 kDa
Usage : 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage : The lysate is shipped with dry ice. Upon receiving, store the sample at -20centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20centigrade. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name ONECUT2 one cut homeobox 2 [ Homo sapiens ]
Official Symbol ONECUT2
Synonyms ONECUT2; one cut homeobox 2; one cut domain, family member 2; one cut domain family member 2; OC 2; onecut 2; HNF-6-beta; transcription factor ONECUT-2; hepatocyte nuclear factor 6-beta; ONECUT-2 homeodomain transcription factor; OC2; OC-2; MGC120377; MGC120378;
Gene ID 9480
mRNA Refseq NM_004852
Protein Refseq NP_004843
MIM 604894
UniProt ID O95948
Chromosome Location 18q21.31
Function sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;

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