Recombinant Human OGN, His-tagged

• Cat.No.: OGN-914H
• Product Overview: A DNA sequence encoding the mature form of human Osteoglycin (Ala20 - Phe298) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 20-298 a.a.
• Description: This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene.
• Form: Lyophilized 100 µg of human Osteoglycin in 100 ul of PBS. Carry free.
• Molecular Mass: 19 KD
• AA Sequence: APPTQQDSRII YDYGTDNFEE SIFSQDYEDK YLDGKNIKEK ETVIIPNEKS LQLQKDEAIT PLPPKKENDE MPTCLLCVCL SGSVYCEEVD IDAVPPLPKE SAYLYARFNK IKKLTAKDFA DIPNLRRLDF TGNLIEDIED GTFSKLSLLE ELSLAENQLL KLPVLPPKLT LFNAKYNKIK SRGIKANAFK KLNNLTFLYL DHNALESVPL NLPESLRVIH LQFNNIASIT DDTFCKANDT SYIRDRIEEI RLEGNPIVLG KHPNSFICLK RLPIGSYF
• Purity: >95% in SDS-PAGE gel
• Applications: ELISA
• Storage: Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at -20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Reconstitution: Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL. Allow to set at least 30 minutes at 4 °C, mix well.

Gene Information

• Gene Name: OGN osteoglycin [ Homo sapiens (human) ]
• Official Symbol: OGN
• Synonyms: OGN; OG; OIF; SLRR3A; osteoglycin; mimecan; mimecan proteoglycan; osteoinductive factor; corneal keratan sulfate proteoglycan
• Gene ID: 4969
• mRNA Refseq: NM_014057
• Protein Refseq: NP_054776
• MIM: 602383
• UniProt ID: P20774
• Chromosome Location: 9q22
• Pathway: Glycosaminoglycan metabolism; Keratan sulfate biosynthesis; MPS I - Hurler syndrome
• Function: growth factor activity; protein binding