Recombinant Human OCLN Protein, His-tagged

• Cat.No.: OCLN-2523H
• Product Overview: Recombinant Human OCLN Protein (Lys266-Thr522) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.8 kDa
  Accurate Molecular Mass: 42 kDa
• Protein length: Lys266-Thr522
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: OCLN occludin [ Homo sapiens (human) ]
• Official Symbol: OCLN
• Synonyms: OCLN; occludin; tight junction protein occludin TM4 minus; tight junction protein occludin; BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277
• Gene ID: 100506658
• mRNA Refseq: NM_001205254
• Protein Refseq: NP_001192183
• MIM: 602876
• UniProt ID: Q16625