Recombinant Human NUDT10, His-tagged

• Cat.No.: NUDT10-982H
• Product Overview: Recombinant human NUDT10 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography(1-164aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NUDT10, also known as APS2 or DIPP3α, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8.5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
• Molecular Weight: 19.5 kDa (172aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDPKHRTYVYVLTV TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT NGNSMAPSSP DSDPLEHHHH HH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NUDT10 nudix (nucleoside diphosphate linked moiety X)-type motif 10 [ Homo sapiens ]
• Synonyms: NUDT10; APS2; DIPP3a; hDIPP3alpha; diphosphoinositol polyphosphate phosphohydrolase 3-alpha; hAps2; DIPP3-alpha; DIPP-3-alpha; nudix motif 10; OTTHUMP00000023298; nucleoside diphosphate-linked moiety X motif 10; diadenosine 5",5""-P1,P6-hexaphosphate hydrolase 3-alpha; diphosphoinositol polyphosphate phosphohydrolase type 3 alpha; NP_694853.1; EC 3.6.1.52
• Gene ID: 170685
• mRNA Refseq: NM_153183
• Protein Refseq: NP_694853
• MIM: 300527
• UniProt ID: Q8NFP7
• Chromosome Location: Xp11.23
• Function: diphosphoinositol-polyphosphate diphosphatase activity; hydrolase activity; metal ion binding