Recombinant Human Nucleosome, His-tagged, Biotinylated

• Cat.No.: NUC-002
• Product Overview: Biotinylated Recombinant Human Nucleosome Protein, fused to N-terminal His-tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa.
• Form: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol.
• Molecular Mass: 113.8 kDa
• Applications: Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E
• Notes: The concentration of this product is determined by Bradford and represents only the protein concentration.
• Stability: At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1-0.5%).
• Storage: Stable at -80°C for at least 6 months from date of receipt, when stored as directed
• Concentration: 1.01 mg/ml

Gene Information

• mRNA Refseq: NM_033445, NM_003528, NM_003532, NM_003549
• UniProt ID: Q7L7L0, Q16778, P68431, P62805