Recombinant Human nuclear receptor subfamily 1, group H, member 2 Protein, StrepII tagged

• Cat.No.: NR1H2-161H
• Product Overview: Recombinant human NR1H2 protein (P55055-1) (Gly 210-Glu 460) with a Strep II tag at the C-terminus was expressed E. coli.

Specification

• Description: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex.
• Source: E. coli
• Species: Human
• Tag: C-StrepII
• Protein length: 210-460aa
• Molecular Mass: 30.5 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: NR1H2 nuclear receptor subfamily 1, group H, member 2 [ Homo sapiens (human) ]
• Official Symbol: NR1H2
• Synonyms: NR1H2; nuclear receptor subfamily 1, group H, member 2; ubiquitously expressed nuclear receptor, UNR; oxysterols receptor LXR-beta; liver X receptor beta; LXR b; NER; NER I; RIP15; LX receptor beta; nuclear receptor NER; liver X nuclear receptor beta; nuclear orphan receptor LXR-beta; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor; UNR; LXRB; LXR-b; NER-I; FLJ17564
• Gene ID: 7376
• mRNA Refseq: NM_001256647
• Protein Refseq: NP_001243576
• MIM: 600380
• UniProt ID: P55055