Recombinant Human NR1H2 protein, His-tagged

• Cat.No.: NR1H2-1399H
• Product Overview: Recombinant Human NR1H2 protein(NP_001243576.2)(Leu219~Glu460) was fused to His-tagged and expressed in E. coli.

Specification

• Description: The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 32kDa(Analysis of differences refer to the manual)
• Protein length: Leu219~Glu460
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Usage: Reconstitute in PBS or others.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NR1H2
• Official Symbol: NR1H2
• Synonyms: NR1-H2; UNR; LXR-B; NER; NER-I; RIP15; Nuclear Receptor Subfamily 1,Group H,Member 2; Ubiquitously-Expressed Nuclear Receptor; Oxysterols receptor LXR-beta
• Gene ID: 7376
• mRNA Refseq: NM_001256647.2
• Protein Refseq: NP_001243576.2