Recombinant Human Nth Endonuclease III-Like 1 (E. coli), His-tagged

• Cat.No.: NTHL1-2488H
• Product Overview: Recombinant human NTHL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 36.6 kDa.

Specification

• Description: NTHL1 is one of the enzyme that has been shown to act on a large number of oxidatively damaged DNA sites. It has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. It acts on a damaged strand, 5" from the damaged site and incises damaged DNA at cytosines, thymines and guanines.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
• Molecular Mass: 36.6 kDa (332aa), confirmed by MALDI-TOF.
• Protein length: 1-312aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL
• Purity: >80% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NTHL1 nth endonuclease III-like 1 (E. coli) [ Homo sapiens ]
• Official Symbol: NTHL1
• Synonyms: NTHL1; nth endonuclease III-like 1 (E. coli); nth (E.coli endonuclease III) like 1; endonuclease III-like protein 1; NTH1; OCTS3
• Gene ID: 4913
• mRNA Refseq: NM_002528
• Protein Refseq: NP_002519
• MIM: 602656
• UniProt ID: P78549
• Chromosome Location: 16p13.3
• Pathway: Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; Cleavage of the damaged pyrimidine, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: 4 iron, 4 sulfur cluster binding; DNA N-glycosylase activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; double-stranded DNA binding; endonuclease activity; hydrolase activity, acting on glycosyl bonds; lyase activity; metal ion binding; protein binding