Recombinant Human NTAN1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NTAN1-6248H
• Product Overview: NTAN1 MS Standard C13 and N15-labeled recombinant protein (NP_775745) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Molecular Mass: 34.7 kDa
• AA Sequence: MPLLVEGRRVRLPQSAGDLVRAHPPLEERARLLRGQSVQQVGPQGLLYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIMNSIKSFSDHAQCGRLEVHLVGGFSDDRQLSQKLTHQLLSEFDRQEDDIHLVTLCVTELNDREENENHFPVIYGIAVNIKTAEIYRASFQDRGPEEQLRAARTLAGGPMISIYDAETEQLRIGPYSWTPFPHVDFWLHQDDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGNKALLYKKNEDGLWEKISSPGSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NTAN1 N-terminal asparagine amidase [ Homo sapiens (human) ]
• Official Symbol: NTAN1
• Synonyms: NTAN1; N-terminal asparagine amidase; protein N-terminal asparagine amidohydrolase; PNAA; PNAD; protein NTN-amidase; protein N-terminal Asn amidase; N-terminal asparagine amidohydrolase; protein NH2-terminal asparagine deamidase; protein NH2-terminal asparagine amidohydrolase; DKFZp666E058
• Gene ID: 123803
• mRNA Refseq: NM_173474
• Protein Refseq: NP_775745
• MIM: 615367
• UniProt ID: Q96AB6