Recombinant Mouse Ntan1 Protein, Myc/DDK-tagged

• Cat.No.: Ntan1-4518M
• Product Overview: Purified recombinant protein of mouse full-length N-terminal Asn amidase (Ntan1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene functions in a step-wise protein degradation process through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asparagine residues on proteins to produce N-terminal L-Aspartate. L-Aspartate substrates are subsequently conjugated to L-Arginine, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Mice with a knock-out of this gene are viable, fertile, and outwardly normal, but show impairments in spontaneous activity and spatial memory, relative to their wild-type counterparts. Alternative splicing results in multiple transcript variants.
• Molecular Mass: 34.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ntan1 N-terminal Asn amidase [ Mus musculus (house mouse) ]
• Official Symbol: Ntan1
• Synonyms: NTAN1; N-terminal Asn amidase; protein N-terminal asparagine amidohydrolase; PNAA; PNAD; protein NTN-amidase; protein N-terminal Asn amidase; N-terminal asparagine amidohydrolase; asparagine-specific N-terminal amidase; protein NH2-terminal asparagine deamidase; protein NH2-terminal asparagine amidohydrolase
• Gene ID: 18203
• mRNA Refseq: NM_010946
• Protein Refseq: NP_035076
• UniProt ID: Q64311