Recombinant Human NRAS 293 Cell Lysate

• Cat.No.: NRAS-3702HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC202681, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NRAS neuroblastoma RAS viral (v-ras) oncogene homolog [ Homo sapiens ]
• Official Symbol: NRAS
• Synonyms: NRAS; neuroblastoma RAS viral (v-ras) oncogene homolog; GTPase NRas; N ras; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; NS6; ALPS4; N-ras; NRAS1
• Gene ID: 4893
• mRNA Refseq: NM_002524
• Protein Refseq: NP_002515
• MIM: 164790
• UniProt ID: P01111
• Chromosome Location: 1p13.2
• Pathway: ARMS-mediated activation, organism-specific biosystem; Activation of RAS in B Cells, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: GTP binding; GTPase activity; nucleotide binding