Active Recombinant Human NRAS Protein, His-tagged

• Cat.No.: NRAS-01H
• Product Overview: The wild-type human N-Ras protein has been produced in a bacterial expression system. The recombinant protein contains six histidine residues at its amino terminus (His-tag).

Specification

• Species: Human
• Source: Bacteria
• Tag: His
• Description: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
• Form: White lyophilized powder
• Bio-activity: The biological activity of N-Ras can be determined from the ability of the RasGRF1 exchange domain (Ras-GRF1-ExD) to catalyze the exchange of GDP for GTP on N-Ras. A standard biological assay for monitoring the biological activity of N-Ras is an exchange assay utilizing the 2× Exchange Buffer from the RhoGEF exchange assay kit and the human RasGRF GEF domain.
• Molecular Mass: 25 kDa
• Purity: Protein purity is determined by scanning densitometry of Coomassie Blue-stained protein on a 4-20% polyacrylamide gradient gel. His tagged N-Ras protein was determined to be > 90% pure.
• Applications: Study of N-Ras exchange activity with different GEFs, Identification of N-Ras exchange factors (GEFs), Positive control for GEF studies, Biochemical characterization of N-Ras protein interactions, Western blot standard
• Stability: The protein is stable for six months if stored at -70 centigrade. The protein should not be exposed to repeated freezethaw cycles. The lyophilized protein is stable at 4 centigrade desiccated (<10% humidity) for one year.
• Storage: In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration, aliquoted into "experiment-sized" amounts, snap frozen in liquid nitrogen, and stored at -70 centigrade.
• Reconstitution: Before reconstitution, briefly centrifuge to collect the product at the bottom of the tube. The protein should be reconstituted to 5 mg/mL with the addition of 20 μL of Milli-Q water (100 μg size). When reconstituted, the protein will be in the following buffer: 50 mM Tris pH 7.5, 50 mM NaCl, 0.5 mM MgCl2, 5% (w/v) sucrose, and 1% (w/v) dextran.

Gene Information

• Gene Name: NRAS NRAS proto-oncogene, GTPase [ Homo sapiens (human) ]
• Official Symbol: NRAS
• Synonyms: NRAS; NRAS proto-oncogene, GTPase; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; GTPase NRas; N-ras protein part 4; neuroblastoma RAS viral (v-ras) oncogene homolog; neuroblastoma RAS viral oncogene homolog; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; EC 3.6.5.2
• Gene ID: 4893
• mRNA Refseq: NM_002524
• Protein Refseq: NP_002515
• MIM: 164790
• UniProt ID: P01111