Recombinant Human NR4A3 293 Cell Lysate

• Cat.No.: NR4A3-3706HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for nuclear receptor subfamily 4, group A, member 3 (NR4A3), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NR4A3 nuclear receptor subfamily 4, group A, member 3 [ Homo sapiens ]
• Official Symbol: NR4A3
• Synonyms: NR4A3; nuclear receptor subfamily 4, group A, member 3; nuclear receptor subfamily 4 group A member 3; CHN; CSMF; MINOR; NOR1; nuclear hormone receptor NOR-1; neuron-derived orphan receptor 1; mitogen-induced nuclear orphan receptor; translocated in extraskeletal chondrosarcoma; chondrosarcoma, extraskeletal myxoid, fused to EWS; TEC
• Gene ID: 8013
• mRNA Refseq: NM_006981
• Protein Refseq: NP_008912
• MIM: 600542
• UniProt ID: Q92570
• Chromosome Location: 9q22
• Pathway: Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Hypertrophy Model, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; binding; metal ion binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; thyroid hormone receptor activity; zinc ion binding