Recombinant Human NR0B2 293 Cell Lysate

• Cat.No.: NR0B2-3722HCL

Specification

• Description: Antigen standard for nuclear receptor subfamily 0, group B, member 2 (NR0B2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: NR0B2 nuclear receptor subfamily 0, group B, member 2 [ Homo sapiens ]
• Official Symbol: NR0B2
• Synonyms: NR0B2; nuclear receptor subfamily 0, group B, member 2; nuclear receptor subfamily 0 group B member 2; SHP; nuclear receptor SHP; small heterodimer partner; orphan nuclear receptor SHP; SHP1; FLJ17090
• Gene ID: 8431
• mRNA Refseq: NM_021969
• Protein Refseq: NP_068804
• MIM: 604630
• UniProt ID: Q15466
• Chromosome Location: 1p36.1
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem
• Function: DNA binding; peroxisome proliferator activated receptor binding; protein binding; protein complex binding; protein domain specific binding; protein homodimerization activity; receptor activity; retinoid X receptor binding; steroid hormone receptor activity; thyroid hormone receptor binding; transcription corepressor activity