Recombinant Human NOTCH1 Protein (Ala19-Gln526), HIgG1 Fc-tagged

• Cat.No.: NOTCH1-461H
• Product Overview: Recombinant Human NOTCH1 Protein (NP_060087.3) (Ala19-Gln526) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Ala19-Gln526
• Description: NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL).The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems.The proto-oncogene NOTCH1 is frequently mutated in around 1% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand binding activity. When NOTCH1 is activated by ligand binding, NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.
• Predicted N Terminal: Ala 19
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human NOTCH1 consists of 741 amino acids and predicts a molecular mass of 79.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: NOTCH1 notch 1 [ Homo sapiens ]
• Official Symbol: NOTCH1
• Synonyms: NOTCH1; notch 1; hN1; TAN1
• Gene ID: 4851
• mRNA Refseq: NM_017617
• Protein Refseq: NP_060087
• MIM: 190198
• UniProt ID: P46531