Recombinant Human NODAL Protein, His-tagged

• Cat.No.: NODAL-2494H
• Product Overview: Recombinant Human NODAL Protein (Ala27-Leu347) with a N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala27-Leu347
• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.4 kDa
  Accurate Molecular Mass: 40 kDa
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: NODAL nodal homolog (mouse) [ Homo sapiens (human) ]
• Official Symbol: NODAL
• Synonyms: NODAL; nodal homolog (mouse); nodal, mouse, homolog; nodal homolog; HTX5; MGC138230
• Gene ID: 4838
• mRNA Refseq: NM_018055
• Protein Refseq: NP_060525
• MIM: 601265
• UniProt ID: Q96S42