Recombinant Human NFYA 293 Cell Lysate

• Cat.No.: NFYA-3840HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for nuclear transcription factor Y, alpha (NFYA), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NFYA nuclear transcription factor Y, alpha [ Homo sapiens ]
• Official Symbol: NFYA
• Synonyms: NFYA; nuclear transcription factor Y, alpha; nuclear transcription factor Y subunit alpha; CBF B; HAP2; NF YA; HAP2 CCAAT-binding protein; Transcription factor NF-Y, A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; nuclear transcription factor Y subunit A; CCAAT-binding transcription factor subunit B; CBF-A; CBF-B; NF-YA; FLJ11236
• Gene ID: 4800
• mRNA Refseq: NM_021705
• Protein Refseq: NP_068351
• MIM: 189903
• UniProt ID: P23511
• Chromosome Location: 6p21.3
• Pathway: Activation of Chaperone Genes by ATF6-alpha, organism-specific biosystem; Activation of Chaperones by ATF6-alpha, organism-specific biosystem; Activation of Genes by ATF4, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Diabetes pathways, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem
• Function: DNA binding; protein binding; sequence-specific DNA binding transcription factor activity