Recombinant Human NFKB Inhibitor Interacting Ras-like 1, His-tagged

• Cat.No.: NKIRAS1-5545H
• Product Overview: Recombinant humanNKIRAS1 protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography.

Specification

• Species: Human
• Tag: His
• Description: NKIRAS1, also knownas NF-kappa-B inhibitor-interacting Ras-like protein 1,belongs to the small GTPase superfamily. NKIRAS1 is atypical Ras-like protein that acts as apotent regulator of NF-kappa-B activity by preventing the degradation ofNF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB ismore resistant to degradation. It may act by blocking phosphorylation ofNFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. Itis unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms blockphosphorylation of NFKBIB.
• Form: Liquid. 20mMTris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT.
• Molecular Weight: 23.8 kDa (212aa),confirmed by MALDI-TOF.
• Purity: > 95%by SDS-PAGE
• Concentration: 0.25 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHH SSGLVPRGSH MGKGCKVVVCGLLSVGKTAI LEQLLYGNHT IGMEDCETME DVYMASVETD RGVKEQLHLY DTRGLQEGVE LPKHYFSFADGFVLVYSVNN LESFQRVELL KKEIDKFKDK KEVAIVVLGN KIDLSEQRQV DAEVAQQWAK SEKVRLWEVTVTDRKTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNS EN
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: NKIRAS1
• Pathways: TNF-alpha/NF-kB Signaling Pathway

Gene Information

• Gene Name: NKIRAS1NFKB inhibitor interacting Ras-like 1 [ Homo sapiens ]
• Synonyms: NKIRAS1; NFKBinhibitor interacting Ras-like 1; KBRAS1; kappaB-Ras1; NF-kappa-Binhibitor-interacting Ras-like protein 1; kappa B-ras 1; kappa B-Ras protein1; I-kappa-B-interacting Ras-like protein 1; OTTHUMP00000208334; OTTHUMP00000208335;OTTHUMP00000208336; OTTHUMP00000208337; OTTHUMP00000208338; OTTHUMP00000208339;OTTHUMP00000214794; OTTHUMP00000214795
• Gene ID: 28512
• mRNA Refseq: NM_020345
• Protein Refseq: NP_065078
• MIM: 604496
• UniProt ID: Q9YNS0
• Chromosome Location: 3p24.2