Recombinant Human Neutrophil Cytosolic Factor 4, 40kDa, His-tagged

• Cat.No.: NCF4-7201H
• Product Overview: Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 41.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-339aa
• Description: NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
• Molecular Mass: 41.1 kDa (359aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHMAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Publications:
  Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils (2019)

Gene Information

• Gene Name: NCF4 neutrophil cytosolic factor 4, 40kDa [ Homo sapiens ]
• Official Symbol: NCF4
• Synonyms: NCF4; neutrophil cytosolic factor 4, 40kDa; neutrophil cytosolic factor 4 (40kD); neutrophil cytosol factor 4; neutrophil NADPH oxidase factor 4; p40phox; SH3PXD4; NCF-4; p40-phox; SH3 and PX domain-containing protein 4; NCF; P40PHOX; MGC3810
• Gene ID: 4689
• mRNA Refseq: NM_000631
• Protein Refseq: NP_000622
• MIM: 601488
• UniProt ID: Q15080
• Chromosome Location: 22q13.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leishmaniasis, organism-specific biosystem
• Function: phosphatidylinositol binding; protein binding; protein dimerization activity