Recombinant Human Nei Endonuclease VIII-like 2 (E. coli), His-tagged

• Cat.No.: NEIL2-1963H
• Product Overview: Recombinant humanNEIL2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Endonuclease 8-like2, also known as NEIL2, belongs to a class of DNA glycosylases homologous tothe bacterial Fpg/Nei family. These glycosylases initiate the first step inbase excision repair by cleaving bases damaged by reactive oxygen species andintroducing a DNA strand break via the associated lyase reaction. It hasnegligible or undetectable activity with 8-oxoguanine, thymine glycol,2-hydroxyadenine, hypoxanthine, and xanthine.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Molecular Weight: 39.4 kDa (356aa),confirmed by MALDI-TOF.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml(determined by Bradford assay).
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMPEGPL VRKFHHLVSP FVGQQVVKTG GSSKKLQPAS LQSLWLQDTQ VHGKKLFLRFDLDEEMGPPG SSPTPEPPQK EVQKEGAADP KQVGEPSGQK TLDGSSRSAE LVPQGEDDSE YLERDAPAGDAGRWLRVSFG LFGSVWVNDF SRAKKANKRG DWRDPSPRLV LHFGGGGFLA FYNCQLSWSS SPVVTPTCDILSEKFHRGQA LEALGQAQPV CYTLLDQRYF SGLGNIIKNE ALYRAGIHPL SLGSVLSASR REVLVDHVVEFSTAWLQGKF QGRPQHTQVY QKEQCPAGHQ VMKEAFGPED GLQRLTWWCP QCQPQLSEEP EQCQFS
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: NEIL2

Gene Information

• Gene Name: NEIL2 nei endonucleaseVIII-like 2 (E. coli) [ Homo sapiens ]
• Synonyms: NEIL2; neiendonuclease VIII-like 2 (E. coli); NEH2; NEI2; endonuclease 8-like 2; neilike 2; nei homolog 2; nei-like protein 2; endonuclease VIII-like 2; DNAglycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2
• Gene ID: 252969
• mRNA Refseq: NM_145043
• Protein Refseq: NP_659480
• MIM: 608933
• UniProt ID: Q969S2
• Chromosome Location: 8p23.1
• Pathway: Base excision repair
• Function: DNA-(apurinic orapyrimidinic site) lyase activity; damaged DNA binding; hydrolase activity,hydrolyzing N-glycosyl compounds; lyase activity; metal ion binding; zinc ionbinding