Recombinant Human NCOA1 protein, His-tagged and Biotinylated

• Cat.No.: NCOA1-372H
• Product Overview: The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein does not contain any tag and will be useful for assays where a native untagged protein is desired.
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Molecular Mass: 21.9 kDa
• AA Sequence: SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT
• Purity: greater than 90% homogeneous based on SDS-PAGE analysis
• Unit Definition: 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay.
• Applications: The biotinylated SCR1-RID (627-786) can be used for protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays.
• Storage: Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.

Gene Information

• Gene Name: NCOA1 nuclear receptor coactivator 1 [ Homo sapiens ]
• Official Symbol: NCOA1
• Synonyms: NCOA1; nuclear receptor coactivator 1; bHLHe74; F SRC 1; KAT13A; NCoA 1; RIP160; SRC1; Hin-2 protein; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1; renal carcinoma antigen NY-REN-52; class E basic helix-loop-helix protein 74; F-SRC-1; bHLHe42; MGC129719; MGC129720
• Gene ID: 8648
• mRNA Refseq: NM_003743
• Protein Refseq: NP_003734
• MIM: 602691
• UniProt ID: Q15788
• Chromosome Location: 2p23
• Pathway: Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; Circadian Repression of expression by REV-ERBA, organism-specific biosystem
• Function: DNA binding; androgen receptor binding; chromatin binding; enzyme binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity