Recombinant Human NCK2, His-tagged

• Cat.No.: NCK2-15879H
• Product Overview: Recombinant human NCK2 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: NCK2 is a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptiona splice variants, encoding different isoforms, have been characterized.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 45.3 kDa (403aa) confirmed by MALDI-TOF
• Protein length: 1-380aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTEEVIV IAKWDYTAQQ DQELDIKKNE RLWLLDDSKT WWRVRNAANR TGYVPSNYVE RKNSLKKGSL VKNLKDTLGL GKTRRKTSAR DASPTPSTDA EYPANGSGAD RIYDLNIPAF VKFAYVAERE DELSLVKGSR VTVMEKCSDG WWRGSYNGQI GWFPSNYVLE EVDEAAAESP SFLSLRKGAS LSNGQGSRVL HVVQTLYPFS SVTEEELNFE KGETMEVIEK PENDPEWWKC KNARGQVGLV PKNYVVVLSD GPALHPAHAP QISYTGPSSS GRFAGREWYY GNVTRHQAEC ALNERGVEGD FLIRDSESSP SDFSVSLKAS GKNKHFKVQL VDNVYCIGQR RFHTMDELVE HYKKAPIFTS EHGEKLYLVR ALQ
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NCK2 NCK adaptor protein 2 [ Homo sapiens ]
• Official Symbol: NCK2
• Synonyms: NCK2; NCK adaptor protein 2; cytoplasmic protein NCK2; NCKbeta; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta; GRB4
• Gene ID: 8440
• mRNA Refseq: NM_003581
• Protein Refseq: NP_003572
• MIM: 604930
• UniProt ID: O43639
• Chromosome Location: 2q12
• Pathway: Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem
• Function: cytoskeletal adaptor activity; protein binding; receptor signaling complex scaffold activity