Recombinant Human NCBP2, His-tagged

• Cat.No.: NCBP2-2847H
• Product Overview: Recombinant human NCBP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =20.1 kDa (176aa), confirmed by MALDI-TOF.

Specification

• Cat. No.: NCBP2-2847H
• Description: NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5" cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3"-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NCBP2 nuclear cap binding protein subunit 2, 20kDa [ Homo sapiens ]
• Synonyms: NCBP2; nuclear cap binding protein subunit 2, 20kDa; CBC2; NIP1; CBP20; PIG55; nuclear cap-binding protein subunit 2; OTTHUMP00000208156; OTTHUMP00000208157; OTTHUMP00000208158; NCBP 20 kDa subunit; NCBP interacting protein 1; NCBP-interacting protein 1; 20 kDa nuclear cap-binding protein; cell proliferation-inducing gene 55 protein
• Gene ID: 22916
• mRNA Refseq: NM_001042540
• Protein Refseq: NP_001036005
• MIM: 605133
• UniProt ID: P52298
• Chromosome Location: 3q29
• Pathway: Spliceosome
• Function: RNA 7-methylguanosine cap binding; NOT RNA binding; RNA cap binding; nucleotide binding; protein binding