Recombinant Human NBR1 293 Cell Lysate

• Cat.No.: NBR1-3956HCL

Specification

• Description: Antigen standard for neighbor of BRCA1 gene 1 (NBR1), transcript variant 3 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: NBR1 neighbor of BRCA1 gene 1 [ Homo sapiens ]
• Official Symbol: NBR1
• Synonyms: NBR1; neighbor of BRCA1 gene 1; M17S2, membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); next to BRCA1 gene 1 protein; 1A1 3B; CA125; KIAA0049; protein 1A1-3B; migration-inducing protein 19; neighbor of BRCA1 gene 1 protein; cell migration-inducing gene 19 protein; membrane component chromosome 17 surface marker 2; membrane component, chromosome 17, surface marker 2 (ovarian carcinoma antigen CA125); M17S2; MIG19; 1A1-3B; FLJ55359; FLJ98272
• Gene ID: 4077
• mRNA Refseq: NM_005899
• Protein Refseq: NP_005890
• MIM: 166945
• UniProt ID: Q14596
• Chromosome Location: 17q21.31
• Function: metal ion binding; mitogen-activated protein kinase binding; mitogen-activated protein kinase p38 binding; protein binding; ubiquitin binding; zinc ion binding