Recombinant Human NAE1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NAE1-4720H
• Product Overview: NAE1 MS Standard C13 and N15-labeled recombinant protein (NP_001018170) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 60.2 kDa
• AA Sequence: MAQLGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVVATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens (human) ]
• Official Symbol: NAE1
• Synonyms: NAE1; NEDD8 activating enzyme E1 subunit 1; amyloid beta precursor protein binding protein 1, amyloid beta precursor protein binding protein 1, 59kDa, amyloid beta precursor protein binding protein 1, 59kD, APPBP1; NEDD8-activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit; ula 1; APP-BP1; protooncogene protein 1; proto-oncogene protein 1; amyloid protein-binding protein 1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein-binding protein 1, 59kD; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; HPP1; ula-1; APPBP1; A-116A10.1
• Gene ID: 8883
• mRNA Refseq: NM_001018160
• Protein Refseq: NP_001018170
• MIM: 603385
• UniProt ID: Q13564