Active Recombinant Human NAE1/UBA3
Cat.No. : | NAE1-168H |
Product Overview : | Recombinant human NAE1 (amino acid residues 3-536) and UBA3 (amino acid residues 22-463), was co-expressed in Insect sf21. |
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Species : | Human |
Source : | Insect Cells |
Tag : | Non |
Protein Length : | 3-536;22-463 a.a. |
Description : | SAE1/SAE2 is a SUMO1, 2 and 3 E1 activating enzyme and functions as a heterodimer. Cloning of the human SAE1 and SAE2 genes was first described by Desterro et al. (1999). SAE1 and SAE2 share sequence similarity to the N-terminus and C-terminus of ubiquitin E1 activating enzymes respectively (Desterro et al. 1999). SAE2 harbours the E1-like active cysteine site while SUMO1 transfer to the E2 conjugating enzyme UBE2I requires both of the SAE subunits (Desterro et al. 1999). A crystal structure of the SAE1/SAE2 dimer together with the SUMO1 adenylate has been solved at 2.45 ?ngstr?m resolution (Olsen et al. 2010). |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E1 Thioester NEDD8 Loading Assay: The activity of APP-BP1/UBA3 was validated by loading NEDD8 onto the active cysteine of APP-BP1/UBA3. Incubation of the APP-BP1/UBA3 enzyme in the presence of NEDD8 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the NEDD8 / APP-BP1/UBA3 thioester bond to the reducing agent DTT was confirmed. |
Molecular Mass : | APP-BP1 = 60.46 kDa UBA3 = 49.35 kDa |
Purity : | >98% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name | NAE1 NEDD8 activating enzyme E1 subunit 1 [ Homo sapiens ] |
Official Symbol | NAE1 |
Synonyms | NAE1; NEDD8 activating enzyme E1 subunit 1; amyloid beta precursor protein binding protein 1 , amyloid beta precursor protein binding protein 1, 59kDa , amyloid beta precursor protein binding protein 1, 59kD , APPBP1; NEDD8-activating enzyme E1 regulatory subunit; NEDD8 activating enzyme E1 subunit; ula 1; APP-BP1; protooncogene protein 1; proto-oncogene protein 1; amyloid protein-binding protein 1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein-binding protein 1, 59kD; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; HPP1; ula-1; APPBP1; A-116A10.1; |
Gene ID | 8883 |
mRNA Refseq | NM_001018159 |
Protein Refseq | NP_001018169 |
MIM | 603385 |
UniProt ID | Q13564 |
Chromosome Location | 16q22 |
Pathway | Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; |
Function | contributes_to NEDD8 activating enzyme activity; catalytic activity; nucleotide binding; protein binding; protein heterodimerization activity; |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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