Recombinant Human NAA20 293 Cell Lysate

• Cat.No.: NAA20-3993HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for N(alpha)-acetyltransferase 20, NatB catalytic subunit (NAA20), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NAA20 N(alpha)-acetyltransferase 20, NatB catalytic subunit [ Homo sapiens ]
• Official Symbol: NAA20
• Synonyms: NAA20; N(alpha)-acetyltransferase 20, NatB catalytic subunit; N acetyltransferase 5 , N acetyltransferase 5 (ARD1 homolog, S. cerevisiae) , N acetyltransferase 5 (GCN5 related, putative) , N acetyltransferase 5, ARD1 subunit (arrest defective 1, S. cerevisiae, homolog) , NAT5; N-alpha-acetyltransferase 20; dJ1002M8.1; N acetyltransferase 3 homolog (S. cerevisiae); NAT3; natB catalytic subunit; natB complex subunit NAT5; N-acetyltransferase 3 homolog; N-acetyltransferase 5 (GCN5-related, putative); N-terminal acetyltransferase complex ARD1 subunit; N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae); N-alpha-acetyltransferase 20, NatB catalytic subunit; N-terminal acetyltransferase B complex catalytic subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae, homolog); NAT5
• Gene ID: 51126
• mRNA Refseq: NM_016100
• Protein Refseq: NP_057184
• MIM: 610833
• UniProt ID: P61599
• Chromosome Location: 20p11.23
• Function: N-acetyltransferase activity; peptide alpha-N-acetyltransferase activity; transferase activity, transferring acyl groups