Recombinant Human N-Ethylmaleimide-Sensitive Factor Attachment Protein, Gamma, His-tagged

• Cat.No.: NAPG-6876H
• Product Overview: Recombinant human NAPG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 37.3 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-312aa
• Description: NAPG is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT.
• Molecular Mass: 37.3 kDa (336aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NAPG N-ethylmaleimide-sensitive factor attachment protein, gamma [ Homo sapiens ]
• Official Symbol: NAPG
• Synonyms: NAPG; N-ethylmaleimide-sensitive factor attachment protein, gamma; gamma-soluble NSF attachment protein; gamma SNAP; SNAP-gamma; soluble NSF attachment protein; GAMMASNAP
• Gene ID: 8774
• mRNA Refseq: NM_003826
• Protein Refseq: NP_003817
• MIM: 603216
• UniProt ID: Q99747
• Chromosome Location: 18p11.21
• Function: protein binding