Recombinant Human Myosin Light Chain Kinase, GST-tagged, Active

• Cat.No.: MYLK-355H
• Product Overview: Recombinant human MLCK (1425-1776) was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW = 70 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1425-1776 a.a.
• Description: MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20-kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. Phosphorylation of regulatory light chain of myosin plays an important role in controlling the morphological changes seen during cell division.
• Sequence: 1425-1776.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at -70℃. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: MYLK myosin light chain kinase [ Homo sapiens ]
• Synonyms: MYLK; myosin light chain kinase; KRP; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083; FLJ12216; DKFZp686I10125; OTTHUMP00000180642; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase; EC 2.7.11.18; OTTHUMP00000180643; Telokin; smMLCK; Kinase-related protein; Myosin light chain kinase, smooth muscle
• Gene ID: 4638
• mRNA Refseq: NM_053025
• Protein Refseq: NP_444253
• MIM: 600922
• UniProt ID: Q15746
• Chromosome Location: 3q21
• Pathway: Calcium signaling pathway; Focal adhesion; Regulation of actin cytoskeleton; Vascular smooth muscle contraction
• Function: ATP binding; actin binding; calcium ion binding; calmodulin binding; magnesium ion binding; myosin light chain kinase activity; nucleotide binding; transferase activity