Recombinant Human Myoglobin, His-tagged

• Cat.No.: MB-58H
• Product Overview: Recombinant Human Myoglobin, fused with C terminal His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Myoglobin is a single-chain globular protein of 153 amino acids, containing a heme prosthetic group in the center around which the remaining apoprotein folds. It has eight alpha helices and a hydrophobic core. It has a molecular weight of 16,700 daltons, and is the primary oxygen-carrying pigment of muscle tissues. Unlike the blood-borne hemoglobin, to which it is structurally related, this protein does not exhibit cooperative binding of oxygen, since positive cooperativity is a property of multimeric/oligomeric proteins only. Instead, the binding of oxygen by myoglobin is unaffected by the oxygen pressure in the surrounding tissue. Myoglobin is often cited as having an "instant binding tenacity" to oxygen given its hyperbolic oxygen dissociation curve.
• Form: The protein solution is in 0.05M phosphate buffer containing 0.15M NaCl and 0.09% NaN3 pH 7.5. Filtered through a 0.2μM membrane.
• Molecular Mass: 48.9kDa
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Applications: Western blotting, ELISA
• Storage: Store it under sterile conditions at -20℃ to -106℃ upon receiving.Recommend to aliquot the protein into smaller quantities for optimal storage.

Gene Information

• Gene Name: MB myoglobin [ Homo sapiens ]
• Official Symbol: MB
• Synonyms: MB; myoglobin; PVALB; MGC13548
• Gene ID: 4151
• mRNA Refseq: NM_005368
• Protein Refseq: NP_005359
• MIM: 160000
• UniProt ID: P02144
• Chromosome Location: 22q13.1
• Function: heme binding; metal ion binding; oxygen binding; oxygen transporter activity