Recombinant Human MYLK3 protein, His & T7-tagged

• Cat.No.: MYLK3-8033H
• Product Overview: Recombinant Human MYLK3 aa. (Glu555~Ser786 (Accession # Q32MK0)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Glu555~Ser786
• Description: Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.4kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: MYLK3 myosin light chain kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MYLK3
• Synonyms: MYLK3; myosin light chain kinase 3; MLCK; MLCK2; caMLCK; myosin light chain kinase 3; MLC kinase; cardiac-MLCK; cardiac-MyBP-C associated Ca/CaM kinase; putative myosin light chain kinase 3
• Gene ID: 91807
• mRNA Refseq: NM_001308301.1
• Protein Refseq: NP_001295230.1
• UniProt ID: Q32MK0