Recombinant Human MYBPC3 protein, His & GST-tagged

• Cat.No.: MYBPC3-8230H
• Product Overview: Recombinant Human MYBPC3 aa. (Met1~Ala328 (Accession # Q14896)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Met1~Ala328
• Description: MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 64.8kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: MYBPC3 myosin binding protein C, cardiac [ Homo sapiens (human) ]
• Official Symbol: MYBPC3
• Synonyms: MYBPC3; myosin binding protein C, cardiac; FHC; CMH4; CMD1MM; LVNC10; MYBP-C; myosin-binding protein C, cardiac-type; C-protein, cardiac muscle isoform; truncated cardiac myosin-binding protein C
• Gene ID: 4607
• mRNA Refseq: NM_000256.3
• Protein Refseq: NP_000247.2
• UniProt ID: Q14896