Recombinant Human MUT protein, His & T7-tagged

• Cat.No.: MUT-1678H
• Product Overview: Recombinant Human MUT aa. (Val510~Ser749 (Accession # P22033)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• ProteinLength: Val510~Ser749
• Description: This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.8kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 8.3

Gene Information

• Gene Name: MUT methylmalonyl-CoA mutase [ Homo sapiens (human) ]
• Official Symbol: MUT
• Synonyms: MUT; methylmalonyl-CoA mutase; MCM; methylmalonyl-CoA mutase, mitochondrial; methylmalonyl Coenzyme A mutase; methylmalonyl-CoA isomerase; mutant methylmalonyl CoA mutase; truncated methylmalonyl CoA mutase
• Gene ID: 4594
• mRNA Refseq: NM_000255.3
• Protein Refseq: NP_000246.2
• UniProt ID: P22033