Recombinant Human MSX1 293 Cell Lysate

• Cat.No.: MSX1-4105HCL

Specification

• Description: Antigen standard for msh homeobox 1 (MSX1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: MSX1 msh homeobox 1 [ Homo sapiens ]
• Official Symbol: MSX1
• Synonyms: MSX1; msh homeobox 1; HOX7, msh (Drosophila) homeo box homolog 1 (formerly homeo box 7) , msh homeobox homolog 1 (Drosophila); homeobox protein MSX-1; HYD1; OFC5; homeobox 7; msh homeo box 1; homeobox protein Hox-7; msh homeobox homolog 1; msh homeobox 1-like protein; HOX7; STHAG1
• Gene ID: 4487
• mRNA Refseq: NM_002448
• Protein Refseq: NP_002439
• MIM: 142983
• UniProt ID: P28360
• Chromosome Location: 4p16.2
• Pathway: HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem
• Function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; p53 binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity